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sigV [2019-04-14 15:47:39]
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sigV [2019-04-14 15:47:39]

[SW|RNA polymerase] ECF-type [SW|sigma factor] SigV, required for resistance to lytic enzymes
locus
BSU27120
pI
9.04
mw
19.57 kDa
protein length
166 aa Sequence Blast
gene length
501 bp Sequence Blast
function
resistance to lytic enzymes
product
[SW|RNA polymerase] ECF-type [SW|sigma factor] SigV
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • Gene

    Coordinates
    2,769,850 → 2,770,350

    Phenotypes of a mutant

  • increased sensitivity to lysozyme, this can be suppressed by overexpression of'' [gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]'' [Pubmed|21856855]
  • The protein

    Protein family

  • [SW|Sigma-70 factor family] (according to UniProt)
  • Effectors of protein activity

  • activity is controlled by the interaction with [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|RsiV], proteolytic degradation of [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|RsiV] releases active [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV] [Pubmed|23687273]
  • Structure

  • [PDB|5WUQ] (the [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]-[protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|RsiW] complex, 28% identity for [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]) [pubmed|28319136]
  • Additional information

  • Expression of the [SW|SigV regulon] in increased in ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutants [Pubmed|22362028]
  • Expression and Regulation

    Operons

    genes
    [gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]-[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]-[gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]-[gene|FC37C7A0C0D9F50CA1D44B8D939CF06E210D4B38|yrhK]
    description
    [Pubmed|21856855]

    sigma factors

  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, [Pubmed|21856855], in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]
  • regulation

  • induced by lysozyme ([protein|search|SigV]) [Pubmed|21856855]
  • additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]
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    Biological materials

    Mutant

  • MGNA-A144 (sigV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/144 NBRP B. subtilis, Japan]
  • 1A907 ( ''sigV''::''kan''), [Pubmed|15838020], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A907&Search=1A907 BGSC]
  • BKE27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC
  • BKK27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC
  • labs

  • [SW|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas Wiegert-Dateien/Thomas Wiegert.html Homepage]
  • References

    Reviews

  • 24921931,26901131
  • Original publications

  • 14993308,16274938,17675383,19745567,22362028,23687273,20817771,21926231,21856855,26399770,29069433,30020925,28319136